ارتباط پلی‌مورفیسم Trp64Arg ژن β3-Adrenoreceptor، نمایه توده بدنی و غلظت سرمی لپتین در مطالعه قند و لیپید تهران

    Background & Aim: There are many articles on the association between β3-adrenoreceptor gene polymorphisms and obesity. The aim of this study was to investigate the association between β3-adrenoreceptor gene polymorphism and serum concentration of Leptin with body mass index(BMI). Materials and Methods: The study was cross-sectional. Participants of Tehran Lipid and Glucose Study were divided on the basis of BMI in four groups: BMI<20, 20≤BMI<25, 25≤BMI<30, BMI≥30. Totally 401 subjects(mean age 44±14 years)[197 men(46±14 years), 204 women(42±13 years)] were selected randomly each group including 97, 98, 104 and 102 subjects, respectively. Leptin level was determined by Enzyme immuno assay(EIA) method and FBS, HDL-C, Triglyceride and Total Cholesterol levels were determined by Enzyme colorimetric method. Factors including Body mass index and blood pressure were measured too. A 210 bp fragment of mentioned gene was amplified using PCR and subject's genotyping in Tehran Lipid and Glucose Study population was determined by RFLP(MvaI, restriction enzyme) method. Data were analyzed using the T-test. We used one-way ANOVA test followed by post hoc multiple comparison(Tukey) between four BMI groups and also between two groups, of AA/TA(arg Carrier) and TT(Arg non Carrier). All data were analyzed using SPSS 11.5. Results: The A(Arg) allele frequency was 0.08 among the population and satisfied Hardy-Weinberg equilibrium. Presence of A(Arg) allele was significantly associated with increase of the BMI(AA/TA, 27±6kg/m2 TT, 25±5 kg/m2 P=0.043) and increase serum Leptin level(AA/TA, 30±25mg/dl TT, 23±21 mg/dl P=0.009). Conclusion: These data showed the association between Trp64Arg polymorphism of β3-adrenoreceptor gene with increasing BMI and Leptin level.